immunohistological evaluation of revascularized immature permanent necrotic teeth treated by platelet-rich plasma: an animal investigation
نویسندگان
چکیده
objective: pulp regeneration within the root canal of necrotic teeth is considered an ideal treatment to allow for continued root development and recover teeth vitality. this study aims to evaluate the inductive effect of platelet-rich plasma (prp) on expression of angiogenesis factors and pulpal revascularization of immature necrotic teeth. materials and methods: in this experimental animal study, we randomly divided 28 immature premolars from two mixed breed dogs into four groups, two experimental, negative and a positive control. premolars in negative control group were left intact to develop normally. in the positive control and experimental groups, we removed the pulps and induced pulp necrosis, after which the chambers were sealed. then, we applied the revascularization protocol in the experimental teeth located in the right quadrant. two months later, the same protocol was applied to the left quadrant. the root canals were disinfected by irrigation with sodium hypochlorite (naocl) solution and application a triple antibiotic past. following the induction of a blood clot (bc) inside the canal space, the coronal portion of the canals was assigned to either of two experimental groups: group 1 [bc+prp+ mineral trioxide aggregate (mta)], group 2 (bc+mta). access cavities were sealed with a glass ionomer. the jaws that held the teeth were processed for histologic analysis of newly formed tissue and immunohistochemical evaluation according to vascular endothelial growth factor (vegf) and factor viii expressions in the canals. results: histological analysis demonstrated no significant difference in the formation of new vital tissue inside the root canals between groups1 (42.8%) and 2 (43.5%, p>0.05). based on immunohistochemical evaluation, micro-vessel density (mvd) of the granulation tissues in both groups were similar and were higher compared with the normal pulp. we observed strongly positive expressions of vegf and factor viii in the stromal and endothelial cells, with severe intensity after one month. both factors showed downregulation at three months postoperative. conclusion: prp could not increase the formation of new vital tissue. the immunohistochemical results showed that vegf and factor viii played a pivotal role in the formation of new vessels inside the root canals of immature, non-vital teeth.
منابع مشابه
Immunohistological Evaluation of Revascularized Immature Permanent Necrotic Teeth Treated by Platelet-Rich Plasma: An Animal Investigation
OBJECTIVE Pulp regeneration within the root canal of necrotic teeth is considered an ideal treatment to allow for continued root development and recover teeth vitality. This study aims to evaluate the inductive effect of platelet-rich plasma (PRP) on expression of angiogenesis factors and pulpal revascularization of immature necrotic teeth. MATERIALS AND METHODS In this experimental animal st...
متن کاملUse of platelet-rich plasma for regeneration in non-vital immature permanent teeth: Clinical and cone-beam computed tomography evaluation
Objective This study was performed to assess the clinical and radiological outcomes of a revascularization procedure in immature teeth with apical periodontitis using platelet-rich plasma (PRP). The PRP protocol and conventional revascularization protocol, which used a blood clot as the scaffold, were compared. Methods Thirty non-vital immature permanent teeth were randomly categorized into two...
متن کاملStereological Evaluation of Cell Proliferation following Intrarenal Injection of Platelet-rich Plasma in Gentamicin-treated Rats
Background & objectives: Nephrotoxicity is the most important side effect of gentamicin and is a concern in clinical situations. There are no therapeutic tools to prevent or palliate nephrotoxicity. Platelet-rich plasma (PRP) as a source of growth factors can be considered as a therapeutic tool. Therefore, the aim of this study was to investigate the effects of PRP on proliferation of epithel...
متن کاملEffects of Platelet-Rich Plasma on Cartilage Grafts in Rabbits as an Animal Model
BACKGROUND Cartilage tissue has limited regenerative capacity and the management of cartilage defects has always been a challenging issue. Platelet-rich plasma (PRP) has been recently been used to improve healing of cartilage defects. In the present experiment, we aimed to investigate the effects of PRP on regeneration capacity as well as survival of the cartilage grafts in a rabbit model. M...
متن کاملPlatelet-Rich Fibrin to Induce Apical Closure in an Immature Multi-Rooted Permanent Tooth with Necrotic Pulp: A Case Report
Regenerative endodontic procedures are the emerging techniques for the restoration of functional pulp and dentine. The case report presented here describes the management of a symptomatic mandibular first molar with an immature apex and necrotic pulp in a 9 year old child. The tooth was tender on percussion and palpation and elicited a negative response to sensibility testing. A regenerative en...
متن کاملPlatelet Rich Plasma: Review of Literature
Objective: At present, growth factor-containing products such as enamel matrix derivatives, recombinant bone morphogenetic protein (rh-BMP), recombinant platelet derived growth factor and platelet rich plasma (PRP) have gained increasing attention. PRP is an autologous source of platelet growth factors used to enhance healing of soft and hard tissues. PRP has gained popularity due to its auto...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
cell journalجلد ۱۸، شماره ۳، صفحات ۳۸۹-۳۹۶
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023